产品名称
红细胞裂解缓冲液Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
Application
建议在细胞颗粒中加入1 mL缓冲液(细胞颗粒=1个脾脏或1-2亿个细胞)。 轻轻混合1分钟。 用15-20mL培养基活盐溶液稀释该缓冲液。 在250-500 × g 离心7分钟并弃去上清。 细胞可被稀释并准备计数或融合。 如果裂解不完全，可重复1-4步。
Biochem/physiol Actions
红细胞裂解缓冲液已被开发用于杂交瘤实验中，用于在融合前从小鼠脾细胞悬液中去除红细胞。 在需要从细胞悬液(如全血)中去除红细胞的体系中，它也很有用。
Preparation Note
含8.3 g/L氯化铵的0.01 M Tris-HCl缓冲液。
Other Notes
注：本品用于去除小鼠红细胞。 本品可能不适用于其他动物红细胞的裂解。 该产品在小鼠脾细胞以外的任何应用场合的适用性必须由研究人员确定。
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
John M Furgason et al.
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Next generation sequencing has become a powerful tool in dissecting and identifying mutations and genomic structural variants that accompany tumourigenesis. Sequence analysis of glioblastoma multiforme (GBM) illustrates the ability to rapidly identify mutations that may affect phenotype. Approximately 50% of
Prabhat K Purbey et al.
Immunity, 47(3), 421-434 (2017-09-21)
Environmental insults are often detected by multiple sensors that activate diverse signaling pathways and transcriptional regulators, leading to a tailored transcriptional output. To understand how a tailored response is coordinated, we examined the inflammatory response elicited in mouse macrophages by
Guoxi Yang et al.
Journal of cellular and molecular medicine, 21(12), 3445-3452 (2017-07-01)
Macrophage surface antigen-1 (Mac-1, CD11b/CD18) has been implicated in the regulation of osteoclastogenesis. In the synovial tissues of patients with aseptic loosening after total hip replacement, CD11b was up-regulated, which indicated that CD11b is closely involved in osteolysis around the
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R7757-100ML | 04061836696771 |