R8653
Anti-ROCK-2 antibody produced in rabbit
~2 mg/mL, affinity isolated antibody, buffered aqueous solution
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IP, WB
Citations:
5
Quality Level
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 160 kDa
species reactivity
human, mouse
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): 25-50 μg using Jurkat cell lysate, microarray: suitable, western blot: 2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ROCK2(9475)
mouse ... Rock2(19878)
rat ... Rock2(25537)
General description
ROCK2 is a serine/threonine kinase that regulates actin cytoskeletal framework. Absence of ROCK2 alleviates atherosclerosis, whereas its overexpression has been associated with hepatocellular carcinoma. Genetic mutations in ROCK2 has been linked to the risk of Behcet′s disease . Anti-ROCK-2 recognizes human and mouse ROCK-2 (160 kDa).
Immunogen
The sequence is highly conserved in Xenopus ROCK-2.
synthetic peptide corresponding to amino acids 1371-1388 located at the C-terminus of human ROCK-2, conjugated to KLH. This sequence is identical in rat, mouse and bovine ROCK-2.
Application
The antibody is suitable for use in immunoprecipitation (25-50 μg using Jurkat cell lysate), microarray and western blot (2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line). The antibody may also be used for immunofluorescence (3-6 μg/ml using NIH3T3 cell line).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
ROCK2 Deficiency in Bone Marrow-Derived Cells Leads to Increased Cholesterol Efflux and Decreased Atherosclerosis.
Zhou, Q., et al.
Circulation, doi: 10-doi: 10 (2012)
Wei Chen et al.
Scientific reports, 8(1), 16636-16636 (2018-11-11)
Rho-associated coiled-coil kinase (ROCK)2 targeting down-regulates autoimmune responses in animal models and patients, however the underlying molecular mechanism is still an enigma. We report that ROCK2 binds phosphorylated-STAT3 and its kinase activity controls the formation of ROCK2/STAT3/JAK2 complex and optimal
Association between Rho-kinase (ROCK2) gene polymorphisms and Behcet's disease.
Oguz, E., et al.
Translational Research, doi: 10-doi: 10 (2012)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R8653-200UL | 04061838107190 |