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Merck
CN

RAB0300

小鼠 IL-4 ELISA 试剂盒

for cell and tissue lysates

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关于此项目

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Il4(16189)
Input:
sample type cell lysate
sample type tissue lysate
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.819-200 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Il4(16189)

General description

The Mouse IL-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-4 in cell lysate and tissue lysate.

Immunogen

Recombinant Mouse IL-4

Application

For research use only. Not for use in diagnostic procedures.
Mouse IL-4 ELISA kit has been used for determining the levels of IL-4 in serum sample.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

IL-4 (interleukin 4) is a pleiotropic cytokine. It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.


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产品编号
说明
化学品安全说明书 & 价格

  • ELISA 5X Sample Diluent Buffer (Item D2)
    化学品安全说明书

  • ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    化学品安全说明书

  • 2X Cell Lysis Buffer (Item J)
    化学品安全说明书

  • ELISA Stop Solution (Item I)
    化学品安全说明书

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    化学品安全说明书

  • 20X Wash Buffer (Item B)
    化学品安全说明书

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Flavonoid Glycosides of Polygonum capitatum protect against inflammation associated with Helicobacter pylori infection.
Zhang Shu
PLoS ONE, 10(5) (2015)
Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.
Hasbold J
European Journal of Immunology, 28, 1040-1051 (1998)
Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilonRI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells.
Pawankar R
The Journal of Clinical Investigation, 99, 1492-1492 (1997)



全球贸易项目编号

货号GTIN
RAB0300-1KT04061834961406