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Merck
CN

RAB0500

Human p53 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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关于此项目

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... TP53(7157)
Input:
sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 ng/mL
standard curve range: 0.328-80 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TP53(7157)

General description

Cellular tumor antigen p53 is a tumor-suppressor protein that comprises transactivation at N-terminus, central sequence-specific DNA-binding domain, and regulatory domain at C-terminus. It plays a key role in autophagy regulation. It inhibits tumors and acts as a cellular stress sensor especially during nutrient deprivation, hypoxia, and ribosome dysfunction. The Human p-53 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human p-53 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human p53

Application

Human p53 ELISA Kit has been used for quantifying p53 using enzyme-linked immunosorbent assay (ELISA) in human myeloma RPMI-8226 cells, human colorectal cancer HCT-116 cells, human breast cancer MCF-7 cells and human leukemiaHL-60 cells.
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.


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产品编号
说明
化学品安全说明书 & 价格

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    化学品安全说明书

  • ELISA 5X Assay/Sample Diluent Buffer D (Item K)
    化学品安全说明书

  • ELISA Stop Solution (Item I)
    化学品安全说明书

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    化学品安全说明书

  • 20X Wash Buffer (Item B)
    化学品安全说明书

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

存储类别

10 - Combustible liquids

法规信息

常规特殊物品
低风险生物材料

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Walaa Hamada Abd-Allah et al.
Drug development research, 80(7), 933-947 (2019-07-26)
Novel 1,1-disubstituted cyclohexane-1-carboxamides 6a-h, 7a-e, and 8a-b were designed and synthesized as apoptotic inducers. Cytotoxicity test revealed that some compounds have strong to moderate effect, while others displayed weak action against different cancer cell lines including, MCF-7, HepG2, A549, and
p53 at a glance.
Colleen A Brady et al.
Journal of cell science, 123(Pt 15), 2527-2532 (2010-10-14)
Evaluation of roscovitine anticancer agent as a reference compound for cancer and apoptosis studies
Taha H, et al.
JIMD Reports null



全球贸易项目编号

货号GTIN
RAB0500-1KT04061834963035