RPROB
Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels
登录 查看组织和合同定价。
选择尺寸
变更视图
关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Technique(s):
protein staining: suitable
Usage:
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
Storage temp.:
2-8°C
usage
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
Quality Level
technique(s)
protein staining: suitable
storage temp.
2-8°C
Application
R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.
Other Notes
Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.
仅试剂盒组分
产品编号
说明
- Solution A 500 mL
- Solution B 500 mL
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
存储类别
12 - Non Combustible Liquids
实验方案
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
商品
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| RPROB-1KT | 04061835573967 |