S1629
硫酸脂酶 来源于产气杆菌
Type VI, buffered aqueous glycerol solution, 2-5 units/mg protein (biuret), 10-20 units/mL
别名:
芳基硫酸酯酶, 苯硫酸酶
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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
2-5 units/mg protein (biuret)
Concentration:
10-20 units/mL
type
Type VI
Quality Level
form
buffered aqueous glycerol solution
specific activity
2-5 units/mg protein (biuret)
mol wt
~41 kDa
concentration
10-20 units/mL
foreign activity
β-Glucuronidase ≤10 U/mL
shipped in
wet ice
storage temp.
−20°C
General description
硫酸酯酶包含Cys/Ser-X-Pro-X-Arg基序，保存在它们的活性部位。
Application
产氧菌磺化酶已用于：
- 用作解凝酶处理血浆样品，通过液相色谱串联质谱（LC/MS/MS）分析进行槲皮素定量
- 基于荧光强度的酶活性检测，使用基于活性的探针1
- 处理硫硫醇脂质体，以去除硫脂中的3-O-硫代半乳糖苷头
Biochem/physiol Actions
硫酸酯酶水解硫酸酯键生成无机硫酸盐。微生物硫化酶参与硫的清除，是硫利用的必需酶。它们可能与发病机制有关。来自产气杆菌的市售硫酸盐酶可用作解凝酶，用于从缀合物中去除葡萄糖醛酸盐和硫酸盐部分。硫酸酯酶广泛用于工业和农业。
Physical form
溶于含0.01 M Tris的50%甘油，pH 7.5。
Other Notes
在pH 7.1，37℃条件下，一个单位可在每分钟水解1.0 μmole对硝基苯磷酸盐。
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Toshiyuki Nakamura et al.
Bioscience, biotechnology, and biochemistry, 75(8), 1506-1510 (2011-08-09)
β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as
Aphichat Trakooncharoenvit et al.
European journal of nutrition, 59(4), 1389-1398 (2019-05-13)
To investigate the effects of water-soluble dietary fibers (pectin, soybean fiber, and guar gum) on the bioavailability of quercetin glucoside mixture (Q3GM) comprising quercetin-3-O-glucoside (Q3G, 31.8%) and its glucose adducts. Male Wistar/ST rats were fed test diet containing 0.7% Q3GM
Melanie Glauser et al.
Clinica chimica acta; international journal of clinical chemistry, 430, 125-128 (2014-01-15)
Total (i.e. free+sulfated) metanephrines in plasma is a biomarker for the diagnosis of pheochromocytoma/paraganglioma. Sulfated metanephrines must be completely deconjugated by perchloric acid hydrolysis or sulfatase treatment prior to analytical measurement to enable quantification by current techniques. In this report
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S1629-10UN | 04061836911768 |
| S1629-50UN | 04061832759685 |