S1951
α-2,3-唾液酸转移酶 来源于多杀巴斯德菌
recombinant, expressed in E. coli BL21, ≥2 units/mg protein
别名:
CMP-N-乙酰神经氨酸酯:β-D-半乳糖苷α-(2,3)-N-乙酰神经氨酰转移酶
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关于此项目
Specific activity:
≥2 units/mg protein
Recombinant:
expressed in E. coli BL21
recombinant
expressed in E. coli BL21
Quality Level
form
lyophilized powder
specific activity
≥2 units/mg protein
mol wt
46.4 kDa
shipped in
dry ice
storage temp.
−20°C
General description
唾液酸转移酶属于糖基转移酶家族，具有催化N-乙酰神经氨酸残基转移的能力。 它是一种具有α--2,3-唾液酸转移酶活性、α-2,6-唾液酸转移酶活性、唾液酸酶活性和反式唾液酸酶活性的多功能酶。 它的分子量大小为46.4 kDa，而pI和pH分别为5.94和7.5-8.5。
Biochem/physiol Actions
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac作为一种供体底物转移至包括糖蛋白、糖脂和寡糖在内的受体分子的β-D-半乳糖基-1,4-N-乙酰基-D-氨基葡萄糖末端。
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac转移至受体分子（包括糖蛋白、糖脂和寡糖）的半乳糖基末端。
Physical form
含有Tris-HCl和NaCl的冻干粉
Preparation Note
以0.1毫升至1毫升体积范围内的水调配冻干粉，可以得到1单位/毫升（1毫升水）至10单位/毫升（0.1毫升水）范围的浓度。
Analysis Note
酶活性检测是在37°C条件下含有CMP-Neu-5Ac (1 mM)以及Lac-β−OMU (1 mM) 的Tris-HCl缓冲液(100 mM, pH 8.0)中反应30 min并通过使用带有荧光检测器（325 nm 激发而372 nm 发射）的HPLC而进行的。
Other Notes
在37 °C pH 8.0条件下，一单位将会在没分钟内催化1.0 μmol Neu-5-Ac-α-2,3LacMU从CMP-Neu-5-Ac和Lac-β−OMU的形成。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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相关内容
Glycobiology and Glycoproteomics Brochure
Instructions
Yoshimitsu Kakuta et al.
Glycobiology, 18(1), 66-73 (2007-10-27)
Sialyltransferases are a family of glycosyltransferases that catalyze the transfer of N-acetylneuraminic acid residues from cytidine monophosphate N-acetylneuraminic acid (CMP-NeuAc) as a donor substrate to the carbohydrate groups of glycoproteins and glycolipids as acceptor substrates. We determined the crystal structure
Peng Zhang et al.
Bipolar disorders, 12(8), 786-792 (2010-12-24)
Multiple linkage and association studies have suggested chromosome 8q24 as a promising candidate region for bipolar disorder (BP). We performed a detailed association analysis assessing the contribution of common genetic variation in this region to the risk of BP. We
E V Chandrasekaran et al.
Journal of proteome research, 11(4), 2609-2618 (2012-02-15)
Our previous studies suggest that the α2,3sialylated T-antigen (NeuAcα2,3Galβ1,3GalNac-) and associated glycan structures are likely to be elevated during cancer. An easy and reliable strategy to label mucinous glycans that contain such carbohydrates can enable the identification of novel glycoproteins
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S1951-1UN | 04061836915940 |