shelf life
≥6 mo. (when stored desiccated and protected from light at room temperature, 4 °C or 20 °C.)
Quality Level
technique(s)
protein staining: suitable
fluorescence
λex 300,470 nm; λem 570 nm
General description
SYPRO Orange与SYPRO Red相似，但它颜色更亮一些，背景荧光略高。 对于使用激光激发凝胶扫描仪的用户，推荐使用一种基于氩离子激光的SYPRO Orange仪器。 该染料可由紫外线或宽带照明光有效激发。它不适用于印迹或IEF凝胶中蛋白的染色，并在2维凝胶染色中显示出较低的灵敏度。
SYPRO Orange为：
SYPRO Orange为：
- 高灵敏度。 该染色剂可检测每minigel条带中1-2ng的蛋白质，比考马斯亮蓝或银染色更灵敏。
- 快速。 在不到1小时内完成染色。
- 简单。 电泳后，对凝胶进行染色、冲洗并拍照；不需要固定或脱色步骤
- 与标准实验室设备兼容。 染色蛋白可以用标准的300纳米紫外透照仪或激光扫描仪来观察。
- 物美价廉。 SYPRO Orange染色法较银染法便宜，并且所需时间短。
- 不同蛋白间变化小。 染料与蛋白质周围的SDS包被层相互作用，使不同类型的蛋白质之间的染色比考马斯或银染色更一致。
- 对蛋白的选择性。 SYPRO Orange检测的蛋白质小至6.5 kDa，不染色核酸或脂多糖。 它染色糖基化蛋白。
- 较宽的检测线性范围。 染色条带的荧光强度与超过3个数量级的蛋白量呈线性相关(比考马斯染色或银染色范围更广)。
Application
SYPRO Orange蛋白凝胶染色技术已应用于蛋白质的热变性和热转移实验。它也被用于微分扫描荧光法。
Legal Information
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.
Disclaimer
SYPRO储液应在室温、4°C或20°C下干燥保存并避光。如果储存得当，这些储液可以稳定使用6个月到1年。使用缓冲液或醋酸溶液稀释后的染色试剂可以储存在非常干净、无去污剂的玻璃瓶或塑料瓶中，在4℃下避光保存至少3个月。
存储类别
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
实验方案
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
商品
为满足蛋白分析的多样化需求，Sigma提供多种蛋白质可视化（染色）试剂。EZBlue™和ProteoSilver™专为蛋白质组学而设计，即使在传统的PAGE形式中依然表现出色。
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S5692-50UL | 04061836954741 |
| S5692-500UL | 04061836954734 |