form
buffered aqueous glycerol solution
Quality Level
specific activity
≥100 units/mg protein (Lowry)
mol wt
34.2 kDa
foreign activity
Phospholipase C ≤1%
storage temp.
2-8°C
Gene Information
Bacillus cereus ATCC 10987 ... spH(2751530)
General description
鞘磷脂酶(SMase)是一种水解酶,分子量为34.2 kDa,其活性位点至少包含两个镁原子。
Application
蜡样芽胞杆菌鞘磷脂酶已用于:
- 在研究中分析鞘磷脂酶对细胞系风疹病毒感染性的影响
- 用于体外生成胆固醇晶体的脂蛋白修饰
- 聚集LDL(低密度脂蛋白)
鞘磷脂酶已用于评估肌动蛋白与HIV-1辅助蛋白Nef相互作用的研究中。 鞘磷脂酶也已用于研究X射线散射作为脂质体药物输送系统的质量控制工具的研究中。
Biochem/physiol Actions
鞘磷脂磷酸二酯酶(SMPD)参与包含鞘磷脂的代谢反应。SMPD具有预防结直肠癌和结肠炎的能力。
启动形成基于鞘磷脂的第二信使。激活 MAPK(丝裂原活化蛋白激酶)和 SAPKs(压力激活蛋白激酶);从鞘磷脂生成神经酰胺。
Physical form
溶于50%甘油,含有50 mM Tris-HCl,pH 7.5
Other Notes
在pH 7.4,37℃条件下,1个单位将每分钟水解1.0 μmol TNPAL-鞘磷脂。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S9396-25UN | 04061836961404 |
| S9396-50UN | 04061832759760 |