SAB4200232
Anti-GAD65 Antibody
mouse monoclonal, N-GAD65
别名:
Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform
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关于此项目
Conjugate:
unconjugated
Clone:
N-GAD65, monoclonal
Application:
IHC, IP, WB
Citations:
14
产品名称
Anti-GAD65 antibody , Mouse monoclonal, clone N-GAD65, purified from hybridoma cell culture
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
N-GAD65, monoclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human, mouse, monkey, rat
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)
General description
Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.
Application
Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.
Biochem/physiol Actions
Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
A GABAergic system in airway epithelium is essential for mucus overproduction in asthma
Xiang YY, et al.
Nature Medicine, 13(7), 862-862 (2007)
Optimized purification strategies for the elimination of non-specific products in the isolation of GAD65-specific monoclonal autoantibodies
Jiang W, et al.
F1000Research, 4 (2015)
GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop
Fenalti G, et al.
Nature Structural and Molecular Biology, 14(4), 280-280 (2007)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SAB4200232-200UL | 04061837725142 |