SAB4200724
抗-AGO2抗体,小鼠单克隆
clone AGO2-10, purified from hybridoma cell culture
别名:
抗-Argonaute2, 抗-PAZ Piwi域蛋白(PPD), 抗-eIF2C 2, 抗-真核翻译起始因子2C 2, 抗-精氨酸RISC催化组分2, 抗-蛋白Argonaute 2
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
AGO2-10, monoclonal
Application:
IP
Citations:
11
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
AGO2-10, monoclonal
form
buffered aqueous solution
mol wt
~95 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using extract of HEK-293T cells transfected with human AGO2, immunoprecipitation (IP): 10-20 μg/test using HeLa cells extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AGO2(27161)
General description
抗-AGO2小鼠单克隆抗体（小鼠IgG1同工型）来源于用人AGO2蛋白C-末端区域并与匙孔血蓝蛋白（KLH）偶联的合成肽免疫的BALB/c小鼠骨髓瘤细胞，和脾细胞相互融合而产生的AGO2-10杂交瘤细胞。AGO2也称为蛋白argonaute 2、argonaute RNA诱导的沉默复合物（RISC）催化成分2、真核翻译起始因子2C 2（eIF2C2）和PAZ Piwi域蛋白（PPD）。Argonaute 2（AGO2）属于Argonaute家族。它定位于人类染色体8q24。Ago蛋白定位于体细胞的细胞质，并在细胞质加工体中浓缩。
Immunogen
对应于人AGO2蛋白C末端区域的合成肽，其与KLH偶联。
Application
抗-AGO2小鼠单克隆抗体已被用于免疫印迹和免疫沉淀。
Biochem/physiol Actions
Argonaute 2（AGO2）具有内在的核酸内切酶活性。它能以小RNA向导的方式直接切割mRNA。AGO2可作为RNA诱导的沉默复合物（RISC）的必要催化成分在RNA沉默过程中起核心作用。它对于稳定细胞外体液中外泌体中的循环microRNA（miRNA）中具有重要作用。
Physical form
溶于含有 15 mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水（pH 7.4）溶液中。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Lasse Peters et al.
Molecular cell, 26(5), 611-623 (2007-06-15)
Small regulatory RNAs such as short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi interacting RNAs (piRNAs) have been discovered in the past, and it is becoming more and more apparent that these small molecules have key regulatory functions. Small RNAs
Andrey Turchinovich et al.
Methods in molecular biology (Clifton, N.J.), 1024, 97-107 (2013-05-31)
Circulating microRNAs (miRNAs) have been recently detected in extracellular body fluids and proved themselves as promising biomarkers for a broad spectrum of diseases. The techniques to isolate, detect, and characterize extracellular miRNAs vary significantly from report to report. In this
Jidong Liu et al.
Science (New York, N.Y.), 305(5689), 1437-1441 (2004-07-31)
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced silencing complex. RISC contains two signature components, small interfering RNAs (siRNAs) and Argonaute family proteins. Here, we show that the multiple Argonaute proteins present in mammals are
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SAB4200724-100UL | 04061838037787 |