Quality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
General description
MISSION pLKO.1-puro 对照载体是一种慢病毒质粒载体。该载体不含 shRNA 插入，在使用 MISSION shRNA 文库克隆的实验中用作阴性对照。 这让人们可以检测转染对基因表达的影响，并解读 shRNA 克隆看到的敲低效应。氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。普尔科。1-puro 对照载体以 10μ 提供g 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl。
Application
MISSION® pLKO.1-puro 空载体对照质粒 DNA 已被用于合成和克隆 shRNA（靶向鼠分化簇 36 (CD36)），以生成慢病毒表达载体。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
Legal Information
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
商品
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
相关内容
Instructions
Jacqueline A Brosnan-Cashman et al.
PloS one, 13(9), e0204159-e0204159 (2018-09-19)
Cancers must maintain their telomeres at lengths sufficient for cell survival. In several cancer subtypes, a recombination-like mechanism termed alternative lengthening of telomeres (ALT), is frequently used for telomere length maintenance. Cancers utilizing ALT often have lost functional ATRX, a
Cardiospecific CD36 suppression by lentivirus-mediated RNA interference prevents cardiac hypertrophy and systolic dysfunction in high-fat-diet induced obese mice.
Zhang Y
Cardiovascular Diabetology, 14 (2015)
AMP-activated protein kinase (AMPK)-induced preconditioning in primary cortical neurons involves activation of MCL-1.
Anilkumar U
Journal of Neurochemistry, 124 (2013)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| SHC001 | 04061826600269 |