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SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ 阳性对照质粒 DNA

Green fluorescent protein marker to monitor transduction efficiency

别名:

MISSION® 对照载体

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关于此项目

UNSPSC Code:
41106609
NACRES:
NA.51
MDL number:
MFCD07785395
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
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Quality Level

200

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

General description

MISSION® TurboGFP™对照载体是一种 8347 碱基对的慢病毒质粒载体，其中包含编码 TurboGFP 的基因，由 CMV 启动子控制。TurboGFP 对照载体可用作 MISSION shRNA 文库克隆实验中的阳性转染/转导对照。TurboGFP™是从桡足类羽小角水蚤（Pontellina plumata） 克隆的绿色荧光蛋白 copGFP 的改良变异体。

氨苄青霉素和嘌呤霉素抗生素抗性基因分别用于细菌和哺乳动物细胞的选择性筛选。此外，利用表达载体与相容包装质粒共转染包装细胞(HEK293T)，可生产自灭活复制缺陷病毒颗粒。TurboGFP对照载体为溶于Tris-EDTA(TE)缓冲液的500 ng/μl溶液，含有10 μg质粒DNA。

Application

MISSION® pLKO.1-puro-CMV-TurboGFP ™ 阳性对照质粒 DNA 已被用于构建 GATA2 过表达载体 (oeG2)，方法是在 5′ 端去掉该载体的 GFP 盒。NheI 和 3′PstI 克隆位点，并用全长 GATA2 转录序列替代。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna

Legal Information

使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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货号GTIN
SHC00304061826600313