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SHCLNV

MISSION® shRNA

Lentiviral Particles

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关于此项目

UNSPSC Code:
41106609
Technique(s):
capture ELISA: 106 VP/mL using p24

Quality Level

200

technique(s)

capture ELISA: 106 VP/mL using p24

shipped in

dry ice

storage temp.

−70°C

General description

我们全面的shRNA产品系列包括超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。该文库可用于：基因家族集合：与特定功能类（如激酶和离子通道）相关的基因集合；基因靶标集合，针对靶标基因的shRNA克隆集合；以及单个shRNA克隆。我们的shRNA和慢病毒制造平台利用专门实验室，配备最先进的机器人技术、液体处理和实验室信息管理系统（LIMS），为您的RNAi研究需求提供卓越的质量和服务。
慢病毒颗粒广泛用于转导各种细胞系。MISSION®慢病毒颗粒含有水疱性口炎病毒(VSV-G)假型，可转导细胞范围更大。 此外，该慢病毒颗粒可将shRNA稳定整合到分裂和非分裂细胞的基因组中。 MISSION生物技术团队稳定产出高质量慢病毒，让我们的客户可以直接开展RNAi实验，不用再将宝贵的时间浪费在繁琐的慢病毒制备上。 我们最高的滴度是106 VP/mL。 不同于腺病毒转染需要高度过量病毒，慢病毒转染更为高效，MOI值（感染复数）低至1。

Application

MISSION® shRNA已用于：
  • 转染和转导
  • 构建表达shRNA的稳定细胞系
  • 建立α-辅肌动蛋白-4(ACTN4)敲低细胞系
  • 转导RAW 264.7细胞

Other Notes

想要查看实验方案和应用数据，请访问 sigma.com/shrna。

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
使用本产品需遵守一个或多个 许可协议。

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

高风险级别生物产品--病毒，疫苗等
低风险生物材料

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

商品

利用慢病毒成功转导

获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

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相关内容

单独基因的shRNA

寻找单独基因的shRNA超过150,000个预克隆的shRNA构建体，靶向超过15,000个人类基因和15,000个小鼠基因。

shRNAs for Individual Genes

Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.

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SHCLNV04061838262202