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SRP0360

PLK1 Polo Box Domain active human

Name: PLK1 Polo Box Domain active human
Brand: SIGMA

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

别名:

STPK13, polo-like kinase 1

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关于此项目

NACRES:

NA.32

UNSPSC Code:

12352200

Assay:

≥90% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli

Concentration:

2.9 mg/mL

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属性

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

53 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

2.9 mg/mL

NCBI accession no.

NM_005030

UniProt accession no.

P53350

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PLK1(5347)

说明

General description

Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.

Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2.

Application

PLK1 Polo Box Domain active human has been used in the in vitro binding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQSpTPLNG to test the effect of various inhibitors.

Biochem/physiol Actions

Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors.

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

5030190501

5030190319

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A selective inhibitor of the Polo-box domain of Polo-like kinase 1 identified by virtual screening.

Sara Abdelfatah et al.

Journal of advanced research, 16, 145-156 (2019-03-23)

Polo-like kinase 1 (PLK1), a member of the Polo-like kinase family, plays an important regulatory role in mitosis and cell cycle progression. PLK1 overexpression is correlated with tumourigenesis and poor prognosis in cancer patients. Therefore, the identification of novel compounds

Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes.

Zsofia A Novak et al.

Developmental cell, 37(6), 545-557 (2016-06-22)

Centrosomes and cilia are organized by a centriole pair comprising an older mother and a younger daughter. Centriole numbers are tightly regulated, and daughter centrioles (which assemble in S phase) cannot themselves duplicate or organize centrosomes until they have passed

Crystallization and preliminary X-ray diffraction studies on the human Plk1 Polo-box domain in complex with an unphosphorylated and a phosphorylated target peptide from Cdc25C.

Begoña García-Alvarez et al.

Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 4), 372-375 (2006-04-04)

Polo-like kinase (Plk1) is crucial for cell-cycle progression via mitosis. Members of the Polo-like kinase family are characterized by the presence of a C-terminal domain termed the Polo-box domain (PBD) in addition to the N-terminal kinase domain. The PBD of

全球贸易项目编号

货号	GTIN
SRP0360-100UG	04061837196188