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SRP0415

PKM1 active human

Name: PKM1 active human
Brand: SIGMA

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

别名:

OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle

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关于此项目

化学文摘社编号:

1644286-11-2

NACRES:

NA.32

UNSPSC Code:

12352200

Assay:

≥80% (SDS-PAGE)

Biological source:

human

Recombinant:

expressed in E. coli

Concentration:

1.8 mg/mL

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属性

biological source

human

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

59 kDa

packaging

pkg of 20 μg

manufacturer/tradename

Sigma-Aldrich

storage condition

avoid repeated freeze/thaw cycles

concentration

1.8 mg/mL

technique(s)

cell based assay: suitable

solubility

water: soluble

NCBI accession no.

NM_182470

UniProt accession no.

P14618

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PKM(5315)

说明

General description

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.

Application

PKM1active human has been used in:

cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.
oxidative stress experiments on PKM2.
Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).

Biochem/physiol Actions

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H360D

pcodes

P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

存储类别

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

4050220329

4050210823

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The monomer of pyruvate kinase, subtype M1, is both a kinase and a cytosolic thyroid hormone binding protein.

C Parkison et al.

Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)

Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and

Nuclear Pyruvate Kinase M2 (PKM2) Contributes to Phosphoserine Aminotransferase 1 (PSAT1)-Mediated Cell Migration in EGFR-Activated Lung Cancer Cells.

Rumeysa Biyik-Sit et al.

Cancers, 13(16) (2021-08-28)

An elevated expression of phosphoserine aminotransferase 1 (PSAT1) has been observed in multiple tumor types and is associated with poorer clinical outcomes. Although PSAT1 is postulated to promote tumor growth through its enzymatic function within the serine synthesis pathway (SSP)

全球贸易项目编号

货号	GTIN
SRP0415-20UG	04061837197628