T2949
抗 mTOR 兔抗
1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
抗 FRAP, 抗 SEP, 抗 FKBP-12 雷帕霉素相关蛋白, 抗 RAFT
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IP, WB
Citations:
14
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 289 kDa
species reactivity
rat, human, mouse
concentration
1.0-1.5 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-1.5 μg using 300 μg RIPA extract of human transformed kidney HEK 293T cells, microarray: suitable, western blot: 1:2,000 using whole extracts of rat brain tissue, western blot: 1:500 using mouse brain tissue
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FRAP1(2475)
mouse ... Frap1(56717)
rat ... Frap1(56718)
General description
mTOR 是一种进化上保守的丝氨酸/苏氨酸激酶，属于磷酸肌醇激酶相关激酶 (PIKK) 家族。该激酶通过启动对营养物质（如氨基酸（主要是亮氨酸）、生长因子、有丝分裂原和胰岛素）的翻译来调节细胞生长。mTOR 通过 S6K1 激活和 4E-BP1 抑制介导翻译起始。mTOR 也可以调节一些细胞功能，如肌动蛋白的组织，分泌，膜运输，蛋白质降解，蛋白激酶 C 信号，tRNA 合成和核糖体生物合成。抗 mTOR 抗体对人、小鼠和大鼠 mTOR (289 kDa) 具有特异性。
Immunogen
对应于人 mTOR 氨基酸残基 2433-2450 的合成肽。
相应的序列在小鼠中相同，在大鼠中相差 1 个氨基酸。
Application
抗mTOR 抗体适用于蛋白免疫分析（1:1,000， 使用虹鳟鱼培养物的肌管细胞裂解液）。该抗体也可用于间接免疫荧光、免疫沉淀和微阵列。
此抗体已成功使用的应用，以及相关的同行评审论文如下。
蛋白免疫分析（Western Blotting）（1篇文章）
蛋白免疫分析（Western Blotting）（1篇文章）
Biochem/physiol Actions
抗 mTOR (FRAP) 使用相应的于人 mTOR 的氨基酸 2433-2450 的肽生产。相应的序列在小鼠中相同，在大鼠中相差 1 个氨基酸。该抗体可特异性识别人、小鼠和大鼠 mTOR (289 kDa)，适用于免疫印迹、免疫沉淀和免疫荧光。
Physical form
溶于含有1% BSA和15 mM叠氮化钠作（pH 7.4）的 .01 M磷酸盐缓冲液。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T2949-200UL | 04061837914485 |