Quality Level
usage
30 mL sufficient for 1,000 extractions, sufficient for 1000 reactions
feature
Direct PCR
technique(s)
PCR: suitable
input
crude DNA
shipped in
wet ice
storage temp.
−20°C
General description
组织制备溶液适用于Extract-N-Amp™ 组织PCR试剂盒、REDExtract-N-Amp™ 组织PCR试剂盒、以及SYBR® Green Extract-N-Amp组织PCR试剂盒。本产品旨在对试剂盒中的试剂进行补充，这些试剂包含许多萃取剂。
Application
组织制备溶液已被用于：
- 结合尾巴片段进行基因分型
- 从斑马鱼胚胎/鳍组织中提取基因组 DNA
- 从小鼠胚胎的耳标、尾尖和胚胎卵黄囊中提取DNA。
- 从真菌孢子和菌丝体中提取 DNA
Legal Information
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
实验方案
exrract-N-Amp试剂盒实验方案提供了一种快速的DNA提取方法，可在15分钟内获得PCR样品。优化的PCR ReadyMix能成功产生清洁的扩增。RED上样染料实现样品跟踪。
Extract-N-Amp kit protocol provides rapid DNA extraction yielding PCR-ready samples in 15 minutes with optimized PCR ReadyMix.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.
Soh BS
Cell Research, 24(12), 1420-1432 (2014)
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jessica Wallingford et al.
Frontiers in molecular neuroscience, 10, 268-268 (2017-09-14)
Astrocyte dysfunction has been indicated in many neurodevelopmental disorders, including Fragile X Syndrome (FXS). FXS is caused by a deficiency in fragile X mental retardation protein (FMRP). FMRP regulates the translation of numerous mRNAs and its loss disturbs the composition
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T3073-.3ML | 04061838250131 |
| T3073-30ML | 04061833768112 |
| T3073-3ML | 04061838101266 |