产品名称
抗-γ-微管蛋白 (DQ-19) 兔抗, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 48 kDa
species reactivity
chicken, human
technique(s)
indirect immunofluorescence: 1:500 using methanol-acetone fixed, cultured chicken fibroblasts., western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TUBG1(7283), TUBG2(27175)
General description
微管蛋白(48 kDa)是真核细胞中微管组织中心(MTOC)或中心体中广泛表达且高度保守的蛋白质。它属于微管蛋白超家族的成员,微管蛋白超家族包括α-和β-微管蛋白以及新发现的中心体相关蛋白δ-和ε-微管蛋白。γ发现-微管蛋白是一个包含至少五种其他蛋白质的大型蛋白复合物的成分,并具有与微管直径大致相同的环形结构(γ-微管蛋白环复合物,γ-TuRC)。其丰度不到α-微管蛋白或 β-微管蛋白水平的1%。 γ-微管蛋白与来自各种生物的α-微管蛋白具有28-32%的同一性,与β-微管蛋白具有32-36%的同一性,与δ-和ε微管蛋白具有29-30%的同一性。在α-,β-,γ-,δ-和ε-微管蛋白之间,某些区域(包括那些被认为与GTP)结合的区域)高度保守。
Immunogen
相应于人γ-微管蛋白(氨基酸433-451,N-末端加入赖氨酸)C-末端的合成肽,与KLH偶联。
Application
兔生产的抗γ微管蛋白(DQ-15)抗体已被用于:
- 蛋白质印迹
- 免疫细胞化学
- 免疫荧光
Biochem/physiol Actions
微管的正确组织对于细胞分裂和染色体分离、定向细胞运动、相间细胞质组织和其他细胞骨架功能至关重要。γ-微管蛋白在中心体的微管成核中具有重要作用。与 β-微管蛋白(微管蛋白分子的一半)结合,建立微管极性,使 α-微管蛋白一半暴露在正端。γ-TuRC(γ-微管蛋白环复合物)可结合微管末端,负责介导微管与中心体之间的连接。
Physical form
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T3195-.2ML | 04061838099860 |