跳转至内容
Merck
CN

主要文件

查看所有文档

T6567

Trypsin Protease

Hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues, suitable for mass spectrometry, from Porcine pancreas

别名:

Porcine Trypsin, Trypsin for Mass Spectropetry

登录 查看组织和合同定价。

选择尺寸

变更视图

关于此项目

UNSPSC Code:
12352204
NACRES:
NA.56
EC Number:
232-650-8
MDL number:
MFCD01775455
Biological source:
Porcine pancreas
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

胰蛋白酶 来源于猪胰腺, Proteomics Grade, BioReagent, Dimethylated

biological source

Porcine pancreas

product line

BioReagent

solubility

1 mM HCl: soluble 1 mg/mL, clear, colorless

shipped in

wet ice

storage temp.

2-8°C

General description

胰蛋白酶是一种丝氨酸蛋白酶，通常在生物化学和生物学中用作重要的酶试剂。这种方法产生了适用于蛋白质组学研究的高度纯化的胰蛋白酶产物。蛋白质组学等级非常适合用于溶液和凝胶胰蛋白酶消化。胰蛋白酶是一种丝氨酸蛋白酶，存在于几种脊椎动物的消化系统中。

Application

猪胰脏胰蛋白酶被应用于以下应用中：
  • 凝胶内蛋白消化及MALDI-TOF质谱分析
  • 电喷雾电离质谱(ESI-MS)分析
  • L. 植物杆菌的表面蛋白质组分析
  • 凝胶过滤，超离心，旋转阴影电子显微镜
  • 质谱

Biochem/physiol Actions

由于胰蛋白酶具有高度特异性裂解能力，胰蛋白酶肽数量有限，因此胰蛋白酶通常用于蛋白质组学研究中的肽定位和蛋白质序列研究。它可特异性地水解精氨酸和赖氨酸残基羧基侧的肽键。该酶还具有酯酶和酰胺酶的活性。胰蛋白酶作为一种细胞培养工具。在工业上，它被用来水解致敏蛋白以生产低致敏牛奶。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Still not finding the right product?

Explore all of our products under 胰蛋白酶 来源于猪胰腺

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCL1499
SLCG2226
SLCK2811
SLCJ6728
SLBZ2416

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

实验方案

胰蛋白酶（EC 3.4.21.4）的酶学测定方案

本方案适用于使用Nα-苯甲酰基-L-精氨酸乙酯（BAEE）作为底物测定胰蛋白酶活性。本方案是基于连续分光光度速率测定（A253，光程= 1cm）方案：

Trypsin Assay Procedure (EC 3.4.21.4)

Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.

商品

化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测

化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测

Better Protease Detection | High-Sensitivity Protease Detection Assay

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

查看所有文章
Pauline Floch et al.
Gut pathogens, 6, 20-20 (2014-07-06)
A gamma-glutamyl transpeptidase (GGT) is produced by up to 31% of strains of Campylobacter jejuni isolates. C. jejuni GGT is close to Helicobacter pylori GGT suggesting a conserved activity but unlike the latter, C. jejuni GGT has not been studied
Yusuke Murasawa et al.
The Journal of biological chemistry, 288(40), 29170-29181 (2013-08-22)
Versican G1 domain-containing fragments (VG1Fs) have been identified in extracts from the dermis in which hyaluronan (HA)-versican-fibrillin complexes are found. However, the molecular assembly of VG1Fs in the HA-versican-microfibril macrocomplex has not yet been elucidated. Here, we clarify the role
Daniela M Remus et al.
Journal of bacteriology, 195(3), 502-509 (2012-11-24)
Sortases are transpeptidases that couple surface proteins to the peptidoglycan of Gram-positive bacteria, and several sortase-dependent proteins (SDPs) have been demonstrated to be crucial for the interactions of pathogenic and nonpathogenic bacteria with their hosts. Here, we studied the role
查看所有相关文献

全球贸易项目编号

货号GTIN
T6567-1MG04061835491971
T6567-20UG04061835519132
T6567-5X20UG04061835575015