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Merck
CN

T8778

海藻糖酶 来源于猪肾脏

buffered aqueous glycerol solution, ≥1.0 units/mg protein

别名:

αα-海藻糖-葡萄糖水解酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥1.0 units/mg protein
Concentration:
0.5-10.0 mg/mL protein (BCA)
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form

buffered aqueous glycerol solution

Quality Level

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

General description

海藻糖是大多数昆虫体内的主要血淋巴糖,是入侵病原真菌的主要靶标。
海藻糖酶(Trehalase)是一种糖苷酶,具有多种形式,存在于所有组织中。属于碳水化合物活性酶(CAZy)类中的糖苷水解酶37(GH37)家族成员。

Application

海藻糖已用于:
  • 评价葡萄霜霉病菌感染葡萄藤叶的糖代谢变化
  • 研究海藻糖-6-磷酸介导的生长停滞
  • 定量分析干酪乳杆菌Lactobacillus casei细胞内的海藻糖

Biochem/physiol Actions

海藻糖酶可催化水解一分子海藻糖生成两分子葡萄糖。它介导飞行代谢、蜕皮诱导的几丁质合成和低温耐受。

Physical form

溶液溶于含 1%Triton X-100 和 25 mM 磷酸钾的 50% 甘油,pH 6.5

Other Notes

一个单位将在37°C、pH 5.7 下每分钟将 1.0μmol 海藻糖转化为 2.0 μg葡萄糖(pH 7.5 下测定释放的葡萄糖)。

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow


存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

低风险生物材料

此项目有



历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Growth arrest by trehalose-6-phosphate: an astonishing case of primary metabolite control over growth by way of the SnRK1 signaling pathway
Delatte, T., et al.
Annual review of plant physiology and plant molecular biology, 157, 160-174 (2011)
Wim Schepers et al.
The Journal of biological chemistry, 287(53), 44130-44142 (2012-11-17)
The readdition of an essential nutrient to starved, fermenting cells of the yeast Saccharomyces cerevisiae triggers rapid activation of the protein kinase A (PKA) pathway. Trehalase is activated 5-10-fold within minutes and has been used as a convenient reporter for
Choowong Auesukaree et al.
Journal of bioscience and bioengineering, 114(2), 144-149 (2012-05-15)
For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723



全球贸易项目编号

货号GTIN
T8778-5UN04061837379642
T8778-1UN04061837379635