产品名称
胰蛋白酶抑制剂 来源于大棉豆 (利马豆), Type II-L, crude powder
biological source
Phaseolus limensis (lima bean)
Quality Level
type
Type II-L
form
crude powder
mol wt
9 kDa
solubility
0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
storage temp.
2-8°C
General description
单体的表观分子量约为 9,000 Da,但发生浓缩和 pH 依赖性二聚化。
Application
来自莱豆的胰蛋白酶抑制剂 (利马豆)已经被用于:
- 在人血浆中抑制胰蛋白酶
- 在兔骨骼肌中终止蛋白水解消化
- 在来自美洲牧草盲蝽的唾液腺酶提取物的抑制
- 在 龙舌兰 酶提取物中抑制蛋白水解活性
胰蛋白酶抑制剂已被用作分离弹性蛋白酶类酶的亲和配体。
Biochem/physiol Actions
青豆胰蛋白酶抑制剂属于蛋白酶抑制剂的 Bowman-Birk 家族。分子量为 9 kDa,含有 9 个二硫键,热稳定性高。具有胰蛋白酶和糜蛋白酶结合子域。
Preparation Note
通过修改 Tauber, H 等人编写的生化与分子生物学行业杂志第 179 期,第 1155 页(1949 年)的方法制备。
Analysis Note
1 mg 将抑制 ≥0.8 mg 胰蛋白酶,活性约为 10,000 BAEE 单位/mg 蛋白质。
Other Notes
在25℃、pH 7.6条件下,反应体积为3.2 mL、光程为1 cm时,以BAEE为底物,一单位胰蛋白酶每分钟可产生0.001的 Δa253 。
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
This technical article described the Enzymatic Assay of Trypsin Inhibitor.
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G Bellon et al.
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A serine protease active on insoluble elastin at neutral pH has been isolated from human aortic media employing a Lima-bean trypsin inhibitor - Sepharose column. It is also hydrolyzed Suc (Ala)3 pna and casein but was found inactive against Benzoyl-Tyr-pna
Are cardiovascular and sympathoadrenal effects of human ?new pressor protein? preparations attributable to human coagulation beta-FXIIa?
Papageorgiou PC, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 286(3), H837-H846 (2004)
A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T9378-500MG | 04061833265888 |
| T9378-100MG | 04061835513277 |
| T9378-1G | 04061826745991 |
