grade
Molecular Biology
sterility
non-sterile; 0.2 μm filtered
product line
BioReagent
form
solution
suitability
suitable for gel electrophoresis (after dilution to working concentration)
foreign activity
Protease, none detected, RNAse, none detected
SMILES string
CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key
HGEVZDLYZYVYHD-UHFFFAOYSA-N
Application
Tris乙酸盐-EDTA缓冲液已被用于在DNA琼脂糖凝胶电泳过程中制备琼脂糖凝胶。
TAE流动缓冲液是DNA琼脂糖凝胶电泳最常用的缓冲液，也可用于非变性RNA琼脂糖凝胶电泳。 双链DNA在TAE中比在其他缓冲液中运行更快，但也可能在延长的电泳过程中耗尽。 在延长的电泳过程中，缓冲液循环或缓冲液更换可以弥补较低的缓冲容量。 浓缩TAE缓冲液稀释后得到1× TAE缓冲液，加入40 mM醋酸三酯和1 mM EDTA，pH 8.3。 1× TAE缓冲液用于琼脂糖凝胶和作为流动缓冲液。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。
Preparation Note
用 18 兆欧水制备的溶液
由经过生物技术性能认证的Trizma碱(T6066)和分子生物学试剂EDTA二钠盐(E5134)制备而成。溶液也含有醋酸(A6283)；粉状混合物含Trizma醋酸(T1258)。
Other Notes
0.4M Tris 乙酸盐，pH 约为 8.3，含有 0.01M EDTA。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries
Quan J and Tian J
Nature Protocols, 6(2), 242-242 (2011)
Yuksel Temiz et al.
Scientific reports, 8(1), 10603-10603 (2018-07-15)
The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for
Joseph P Torella et al.
Nature protocols, 9(9), 2075-2089 (2014-08-08)
Recombination-based DNA construction methods, such as Gibson assembly, have made it possible to easily and simultaneously assemble multiple DNA parts, and they hold promise for the development and optimization of metabolic pathways and functional genetic circuits. Over time, however, these
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T9650-1L | 04061838260475 |
| T9650-4L | 04061838260482 |