U5378
尿素
powder, BioReagent, Molecular Biology, suitable for cell culture
别名:
碳酰二胺, 碳酰胺
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关于此项目
线性分子式:
NH2CONH2
化学文摘社编号:
分子量:
60.06
UNSPSC Code:
12352111
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-315-5
Beilstein/REAXYS Number:
635724
MDL number:
Assay:
≥98%
Grade:
Molecular Biology
Technique(s):
cell culture | mammalian: suitable
grade
Molecular Biology
Quality Level
product line
BioReagent
assay
≥98%
form
powder
technique(s)
cell culture | mammalian: suitable
mp
132-135 °C (lit.)
solubility
H2O: soluble 480 mg/mL, clear, colorless (8M)
density
1.335 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm
cation traces
Cu: ≤1 ppm, Fe: ≤5 ppm, Pb: ≤2 ppm
absorption
≤0.06 at 260 at 6 M, ≤0.06 at 280 at 6 M
foreign activity
DNase, NICKase, RNase and protease, none detected
SMILES string
NC(N)=O
InChI
1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
InChI key
XSQUKJJJFZCRTK-UHFFFAOYSA-N
Gene Information
human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)
Application
- 尿素已可用于蛋白质重悬缓冲液和蛋白质变性。
- 它已可用于通过含有尿素的聚丙烯酰胺凝胶分离蛋白质。
- 它被用作研究不同浓度尿素和不同pH值下未折叠蛋白的构象。
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Construction and transfection of a ribozyme targeting human caspase-3.
Cronin D et al.
Anticancer Research, 24, 425-425 (2004)
Labeling and identification of direct kinase substrates.
Carlson SM and White FM
Science Signaling, 5, pl3-pl3 (2012)
Structure and properties of native and unfolded lysing enzyme from T. harzianum: Chemical and pH denaturation.
Bey H et al.
International Journal of Biological Macromolecules, 92, 860-860 (2016)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| U5378-500G | 04061835550449 |
| U5378-100G | 04061835522675 |
| U5378-1KG | 04061835550432 |
| U5378-5KG | 04061835550456 |