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W1878

Williams’ 培养基E

With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

别名:

Williams’ E medium

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关于此项目

UNSPSC Code:
12352207
NACRES:
NA.75
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产品名称

Williams′ 培养基E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

General description

Williams培养基E由Williams和Gunn在Williams培养基D的基础上改良而成。培养基专用于支持成人肝脏上皮细胞的长期细胞培养。它是改良型最低必需培养基(MEM)，改变了葡萄糖和氨基酸的含量。

Application

Williams培养基E已用于培养人肝细胞癌(HCC)细胞和肝细胞。

Preparation Note

添加 0.292g/L L-谷氨酰胺。

Other Notes

酚红会干扰一些细胞在低密度或克隆密度下的生长。在使用干细胞或低密度细胞时，请使用这款William's E。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL6338
RNBK8801
RNBK2846
RNBJ7505
RNBJ3314

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(W1878) - Formulation

Instructions

Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
Dariusz Ratman et al.
Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
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全球贸易项目编号

货号GTIN
W1878-6X500ML04061837488733
W1878-500ML04061837488726