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SYBR® Green Extract-N-Amp™组织PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

别名:

Tissue direct PCR kit, Tissue direct real-time PCR

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关于此项目

NACRES:
NA.55
UNSPSC Code:
41106303
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Quality Level

200

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

feature

Direct PCR, dNTPs included, hotstart

technique(s)

PCR: suitable

input

crude DNA

storage temp.

−20°C

General description

The SYBR® Green Extract-N-Amp™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Application

SYBR® Green Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and genotyping.

Biochem/physiol Actions

DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp™ SYBR® Green PCR ReadyMix™ and user-provided PCR primers.

Features and Benefits

  • Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR)
  • Fast – tissue to qPCR in 15 minutes
  • Convenient – no long enzymatic digestions and no column purifications
  • Simple – rapid, easy-to-follow protocol
  • Sensitive – specially formulated HOTSTART® SYBR® Green PCR ReadyMix™ for highly specific PCR amplification and quantitation of genomic DNA
  • Safe – no organic extraction with hazardous chemicals

Other Notes

更多信息，请见www.sigma-aldrich.com/extract-n-amp。
试剂盒含有Extraction Solution、Neutralization Solution、Tissue Preparation Solution和Extract-N-Amp SYBR Green PCR ReadyMix™。Extract-N-Amp SYBR Green PCR ReadyMix为2X反应混合物，其中含有 SYBR Green、缓冲液、盐、dNTP、Taq聚合酶和 JumpStart™ Taq 抗体。此试剂盒专为结合提取试剂使用进行了优化，其中含有适用于热启动PCR、可增强特异性的 JumpStart Taq抗体，以及可用作实时定量PCR的非特异性报告标志物的SYBR Green I。

Legal Information

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056和6,171,785。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。其他专利声明下的任何权利（如美国专利号6,814,934号下的设备和系统专利声明）、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。

依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

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Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

存储类别

10 - Combustible liquids

wgk

WGK 3

法规信息

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实验方案

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

商品

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Linda Doan et al.
Journal of visualized experiments : JoVE, (11)(11), doi:10-doi:10 (2008-12-11)
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
Haroldo A Toque et al.
PloS one, 8(8), e72277-e72277 (2013-08-27)
Elevated arginase (Arg) activity is reported to be involved in diabetes-induced vascular endothelial dysfunction. It can reduce L-arginine availability to nitric oxide (NO) synthase (NOS) and NO production. Akita mice, a genetic non-obese type 1 diabetes model, recapitulate human diabetes.
Modesto Rojas et al.
PloS one, 8(12), e84357-e84357 (2013-12-21)
Diabetic retinopathy, a major cause of blindness, is characterized by increased expression of vascular endothelial growth factor (VEGF), leukocyte attachment to the vessel walls and increased vascular permeability. Previous work has shown that reactive oxygen species (ROS) produced by the
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货号GTIN
XNATRG-1KT04061837549991