Anti-SHP-1/PTPN6 Antibody, clone 1D20 ZooMAb^® Rabbit Monoclonal

Tyrosine-protein phosphatase non-receptor type 6 (UniProt: P29350; also known as EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1) is encoded by the PTPN6 (also known as HCP, PTP1C) gene (Gene ID: 5777) in human. Protein tyrosine phosphorylation plays a critical role in regulating cell growth, differentiation, and activation of terminally differentiated cells. The level of phosphorylation results from a balance between the activity of protein tyrosine kinases and protein tyrosine phosphatases. SHP-1 is a monomeric protein phosphatase that is mainly expressed in hematopoietic cells and contains two SH2 domains. Its SH2 domains interact with other cellular components to modulate its own phosphatase activity against interacting substrates. The SH2 domains are critical for SHP-1 to bind to ITIM domains in inhibitory immunoreceptors. SHP-1 is localized in the cytoplasm, but it shuttles between the cytoplasm and nucleus via its association with phosphoinositide-dependent Kinase-1 (PDPK1). It modulates signaling by tyrosine phosphorylated cell surface receptors, such as KIT and EGFR. It contains two putative sites of tyrosine phosphorylation in its C-terminal region that can be phosphorylated by both receptor and non-receptor protein tyrosine kinases. SHP-1 is reported to undergo phosphorylation at tyrosine 564 by Lyn that enhances its phosphatase activity. SHP-1 is considered as a tumor suppressor and the main mechanism by which it curbs tumor development and progression is by its ability to attenuate signaling pathways that control cell proliferation, survival, migration, and invasion. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Varone, A., et al. (2020). Front. Oncology. 10; 935; Yoshida, K., et al. (1999). J. Biol. Chem. 274(49); 34663-34668; Li, RY., et al. (1995). EMBO J. 14(11); 2519-2526).

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human SHP-1/PTPN6.

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SHP-1/PTPN6 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SHP-1/PTPN6 in THP-1cell lysate.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected SHP-1/PTPN6 in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SHP-1/PTPN6 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound SHP-1/PTPN6 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 1D20 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects SHP-1/PTPN6. It targets an epitope within 20 amino acids from the N-terminal half.

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.