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Merck
CN

51576-U

Ascentis® Express 90 Å RP-Amide (2 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm UHPLC Column

别名:

Core-shell (SPP) Fused Core polar embedded HPLC column

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关于此项目

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
10 cm × 2.1 mm
Particle size:
2 μm
Matrix active group:
amide, alkyl phase
Pore size:
90 Å pore size
Matrix:
Fused-Core particle platform, superficially porous particle
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产品名称

Ascentis® Express RP-Amide, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel column

Quality Level

agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

1000 bar max. pressure (14500 psi), 60 °C temp. range

technique(s)

LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

120 m2/g

impurities

<5 ppm (metals)

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

amide, alkyl phase

particle size

2 μm

pore size

90 Å pore size

pH

2-9

application(s)

food and beverages

separation technique

reversed phase

General description

Ascentis® Express 2 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on a new Fused-Core® particle design. The fused core partical provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 micron thick porous shell and the small overall particle size of 2-microns. The one-step bonded, end-capped, amide-based polar-embedded stationary phase of Ascentis Express 2 μm RP-Amide provides, a stable, reversed phase packing with decreased hydrophobic character. Ascentis Express 2 μm RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8.

Application


  • A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study demonstrates the application of the Ascentis® Express RP-Amide, 2 μm UHPLC Column for analyzing complex mixtures of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The developed method was validated for precision, accuracy, and robustness, showing significant improvement in separation efficiency and analysis time compared to conventional columns. This method is particularly valuable for researchers focusing on phytochemical analysis and natural product chemistry (Fibigr et al., 2018).

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.


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货号GTIN
51576-U04061838529909