567516-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
别名:
Discovery五氟苯基PFP HPLC色谱柱
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关于此项目
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
15 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
120 Å
Matrix:
silica gel, high purity, spherical particle platform, fully porous particle
产品名称
Discovery ® HS F5 HPLC 色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
material
stainless steel column
Quality Level
agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
15 cm × 4.6 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform, fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
5 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
General description
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
Application
- Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.：利用Discovery® HS F5 HPLC色谱柱出色分离大米中各种砷形态，改进食品安全性分析，保证健康监管合规性(Baba et al., 2014)。
- High-performance liquid chromatographic determination of tiapride and its phase I metabolite in blood plasma using tandem UV photodiode-array and fluorescence detection.：使用Discovery® HS F5 HPLC色谱柱进行硫必利及其代谢物的同步灵敏检测，提供药物监测和药物动力学研究参考价值。体现本品的多用途(Nobilis et al., 2011)。
- Simultaneous quantification of nicotine and metabolites in rat brain by liquid chromatography-tandem mass spectrometry.：利用Discovery® HS F5 HPLC色谱柱准确检测大鼠脑中尼古丁及其代谢产物，为药物成瘾和神经药理学研究提供了重要数据(Vieira-Brock et al., 2011)。
- Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.：用Discovery® HS F5 HPLC色谱柱找出各种糖尿病小鼠生物样本中的生物标志物，贡献分子水平的糖尿病知识(Tsutsui et al., 2011)。
- Practical analytical approach for the identification of biomarker candidates in prediabetic state based upon metabonomic study by ultraperformance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry.：在代谢组学方法中采用Discovery® HS F5 HPLC色谱柱以鉴定糖尿病早期生物标志物，促进预防医学和代谢研究(Tsutsui et al., 2010)。
Features and Benefits
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Milan Nobilis et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(32), 3845-3852 (2011-11-22)
New bioanalytical SPE-HPLC-PDA-FL method for the determination of the neuroleptic drug tiapride and its N-desethyl metabolite was developed, validated and applied to xenobiochemical and pharmacokinetic studies in humans and animals. The sample preparation process involved solid-phase extraction of diluted plasma
Hiroyuki Kataoka et al.
Analytical and bioanalytical chemistry, 405(1), 331-340 (2012-10-16)
We have developed a simple and sensitive method for the simultaneous determination of testosterone (TES), cortisol (CRT), and dehydroepiandrosterone (DHEA) in saliva by automated online in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Discovery HS
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 567516-U | 04061838545633 |