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Merck
CN

V900887

蛋白酶 K 来源于林伯氏白色念球菌

Vetec, reagent grade, powder, ≥30 units/mg protein

别名:

肽链内切酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
EC Number:
254-457-8
MDL number:
Specific activity:
≥30 units/mg protein
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grade

reagent grade

product line

Vetec

form

powder

specific activity

≥30 units/mg protein

mol wt

28.93 kDa

technique(s)

DNA extraction: suitable

foreign activity

Dnase ≤30 units/mg solid

storage temp.

−20°C

Application

在分离DNA和RNA的过程中,对核酸酶的蛋白水解失活起作用。
在分离DNA和RNA的过程中,用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

Biochem/physiol Actions

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族,具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定:pH,缓冲盐,去污剂(SDS)以及温度。 在0.1-0.5% SDS存在的情况下,蛋白酶K可保持活性,在不损害分离DNA完整性的前提下,可以消化DNA制剂中的多种蛋白质和核酸酶。

Other Notes

在pH 7.5、37摄氏度条件下,一单位酶每分钟水解尿素变性的血红蛋白产生的颜色强度,相当于1.0微摩酪氨酸。

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

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