Determination of Chlorogenic acid and Baicalin in compounded Lonucerae Japonicae Flos with Purospher^® STAR RP-18e

Chinese Pharmacopeia method
Dean Duan
Merck
China application lab
Shanghai, China

Compounded Lonucerae Japonicae Flos is a kind of Traditional Chinese Medicine (TCM). It has the effects of heat-clearing and detoxicating, also of cooling blood and detumescence.

In this application, one Chinese pharmacopeia 2020 monograph method was validated using a Purospher^® STAR RP-18 endcapped HPLC column. The samples were first ground, and the active ingredients chlorogenic acid and baicalin were extracted with an aqueous methanol solution. All samples were filtered through Millex^® PTFE syringe filters prior to HPLC-UV analysis.

The limit of detection (LOD) and the limit of quantitation (LOQ) were found to be 0.06 µg/mL and 0.017 µg/kg, respectively, for chlorogenic acid, while the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.29 µg/mL and 0.87 µg/kg, respectively, for baicalin. The method can be used for determination of chlorogenic acid and baicalin in compounded Lonucerae Japonicae Flos.

Determination of chlorogenic acid and baicalin in compounded Lonucerae Japonicae Flos

Chromatograms at 278 nm for chlorogenic acid

Chromatograms at 326 nm for baicalin

Specificity and repeatability – chlorogenic acid and baicalin

Calibration data – chlorogenic acid

Calibration data – baicalin

Conclusion

The Chinese pharmacopeia 2020 monograph method was validated using a using a Purospher^® STAR RP-18 endcapped HPLC column. The method can be used for determination of chlorogenic acid and baicalin in compounded Lonucerae Japonicae Flos.