860336
3,3′,5,5′-Tetramethylbenzidine
≥99%
Synonym(s):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
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About This Item
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
EC Number:
259-364-6
Beilstein/REAXYS Number:
2808541
MDL number:
Assay:
≥99%
Form:
powder
Quality Level
assay
≥99%
form
powder
mp
168-171 °C (lit.)
storage temp.
2-8°C
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
General description
3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Iron oxide nanoparticles: Hidden talent
Perez J M
Nature Nanotechnology, 2(9), 535-535 (2007)
Colorimetric method for determination of chlorine with 3, 3?, 5, 5?-tetramethylbenzidine.
Serrat F B
Talanta, 41(12), 2091-2094 (1994)
Ronit Mazor et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(23), 8571-8576 (2014-05-07)
Nonhuman proteins have valuable therapeutic properties, but their efficacy is limited by neutralizing antibodies. Recombinant immunotoxins (RITs) are potent anticancer agents that have produced many complete remissions in leukemia, but immunogenicity limits the number of doses that can be given
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 860336-1G | 04061835369980 |
| 860336-100MG | 04061833033135 |
| 860336-25G | 04061835369997 |
| 860336-5G | 04061835370009 |