Skip to Content
Merck
CN

Key Documents

View All Documentation

900892

L-Azidohomoalanine hydrochloride

Synonym(s):

AHA

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C4H8N4O2 · HCl
CAS Number:
942518-29-8
Molecular Weight:
180.59
MDL number:
MFCD13184931
UNSPSC Code:
12352209
NACRES:
NA.21
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

powder or crystals

Quality Level

100

reaction suitability

reaction type: click chemistry

mp

139 °C

storage temp.

−20°C

SMILES string

[Cl-].[N+H3][C@@H](CCN=[N+]=[N-])C(=O)O

InChI

1S/C4H8N4O2.ClH/c5-3(4(9)10)1-2-7-8-6;/h3H,1-2,5H2,(H,9,10);1H/t3-;/m0./s1

InChI key

MHHYJRIDKLZZEO-DFWYDOINSA-N

Application

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety. This compound can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. For example the azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS/MS and MALDI-MS analysis.

pictograms

Flame
GHS02

signalword

Danger

hcodes

H242

Hazard Classifications

Self-react. C

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCQ8143
MKCQ0828
MKCN9849
MKCN2591
MKCL8936

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

交联剂选择指南

为您的生化或生物共轭研究选择合适的交联剂可能具有挑战性。我们的选择指南可以帮助您找到最适合您应用的交联剂。

What is Click Chemistry? An Introduction

Explore click chemistry: efficient, high-yield reactions for drug discovery, bioconjugation, and green chemistry with simple conditions and wide applications.

Click Chemistry in Drug Discovery

Explore the principles and applications of click chemistry in drug discovery, highlighting efficient reactions that streamline the synthesis of bioactive compounds.

View All Articles
Expanding the diversity of chemical protein modification allows post-translational mimicry.
van Kasteren S I, et al.
Nature, 446(7139), 1105-1105 (2007)
Teresa Rayon et al.
Science (New York, N.Y.), 369(6510) (2020-09-19)
Although many molecular mechanisms controlling developmental processes are evolutionarily conserved, the speed at which the embryo develops can vary substantially between species. For example, the same genetic program, comprising sequential changes in transcriptional states, governs the differentiation of motor neurons

Global Trade Item Number

SKUGTIN
900892-100MG04061833251362