TissueFab^® bioink kit

Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. Laminin is an extracellular matrix multidomain trimeric glycoprotein and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Fibronectin is a multifunctional glycoprotein which exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. Fibronectin is known to contain integrin binding sequences which facilitate cell-matrix interactions. Fibronectin plays an essential role in multiple biological functions such as, angiogenesis, cell migration and differentiation and wound healing.

The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation Gelatin methacrylate based bioinks have been used to bioprint osteogenic , chondrogenic , hepatic , adipogenic , vasculogenic , epithelial , endothelial , cardiac valve , skin , tumor and other tissues and constructs. Fibronectin has been used in tissue engineering applications for wound healing and angiogenesis , bone repair and studying mechanotransduction.

TissueFab^® - GelMA Fibronectin bioink kit UV/365 features a ready-to-use bioink that is formulated for high cell viability, and printability and is designed for extrusion-based 3D bioprinting and subsequent 365 nm light crosslinking and a solution of Fibronectin to enhance the bioink. The bioink kit can be used with most extrusion-based bioprinters, are biodegradable, and are compatible with human dermal fibroblasts (HDFs) and other diverse cell types. TissueFab^® - GelMA Fibronectin bioink kit UV/365 enables the precise fabrication of 3D cell models and tissue constructs for research in 3D cell biology, tissue engineering, in vitro tissue models, and regenerative medicine. Suitable for final Fibronectin concentrations up to 200 μg/ml.

In addition to fast gelation, the methacrylamide functional group can also be used to control the hydrogel physical parameters such as pore size, degradation rate, and swell ratio. Temporal and spatial control of the crosslinking reaction can be obtained by adjusting the degree of functionalization and polymerization conditions, allowing for the fabrication of hydrogels with unique patterns, 3D structures, and morphologies.Low Endotoxin, low bioburden: Endotoxins have been demonstrated negatively impact cellular growth, morphology, differentiation, inflammation and protein expression. Bioburden is defined as the number of contaminated organisms found in a given amount of material. We test each lot for endotoxins as well as total bioburden (aerobic and fungal) to minimize unwanted interactions. For more information: https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/microbiological-testing/pyrogen-testing/what-is-endotoxin

TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany