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Merck
CN

810255P

Avanti

TopFluor Cholesterol

Avanti Research - A Croda Brand

Synonym(s):

Bodipy Cholesterol; Bdp-Chol

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About This Item

Empirical Formula (Hill Notation):
C36H51BF2N2O
CAS Number:
Molecular Weight:
576.61
MDL number:
NACRES:
NA.25
UNSPSC Code:
12352211
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description

23-(dipyrrometheneboron difluoride)-24-norcholesterol

assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810255P-1mg), pkg of 1 × 10 mg (810255P-10mg), pkg of 1 × 5 mg (810255P-5mg)

manufacturer/tradename

Avanti Research - A Croda Brand

shipped in

dry ice

storage temp.

−20°C

General description

Biomolecules attached to BODIPY, is readily absorbed in the visible light. It results in greater fluorescence quantum yields. It is highly photostable and less sensitive to change in polarity and pH.

Application

TOPFLUORCholesterol is suitable:
  • for cholesterol-uptake assay
  • for BODIPY cholesterol labeling in live cell experiments
  • for the formation of giant Janus liposomes via poly(vinyl alcohol) (PVA)-assisted lipid swelling

Biochem/physiol Actions

BODIPY labelled cholesterol has similar properties to that of the naïve cholesterol. This serves as a cholesterol marker for live cells and aids in trafficking intracellular cholesterol. It might also help in indicating lipid storage diseases.

Packaging

5 mL Amber Glass Screw Cap Vial (810255P-10mg)
5 mL Amber Glass Screw Cap Vial (810255P-1mg)
5 mL Amber Glass Screw Cap Vial (810255P-5mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC
TopFluor is a trademark of Avanti Polar Lipids, LLC


Storage Class

11 - Combustible Solids

wgk

WGK 3



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Articles

Explore full-spectrum fluorophores, fluorescent lipids, and probes for imaging, membrane studies, and labeling with TopFluor™, squaraines, and more.


Frederik W Lund et al.
BMC biophysics, 5, 20-20 (2012-10-20)
Cholesterol is an important membrane component, but our knowledge about its transport in cells is sparse. Previous imaging studies using dehydroergosterol (DHE), an intrinsically fluorescent sterol from yeast, have established that vesicular and non-vesicular transport modes contribute to sterol trafficking
Maarit Hölttä-Vuori et al.
Traffic (Copenhagen, Denmark), 9(11), 1839-1849 (2008-07-24)
Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. In this study, we employed atomistic simulations and experiments to characterize a cholesterol compound with
Robert M Littleton et al.
BMC complementary and alternative medicine, 12, 105-105 (2012-07-25)
Consumers are increasingly turning to plant-based complementary and alternative medicines to treat hypercholesterolemia. Many of these treatments are untested and their efficacy is unknown. This multitude of potential remedies necessitates a model system amenable to testing large numbers of organisms



Global Trade Item Number

SKUGTIN
810255P-1MG04061835185634
810255P-10MG04061835185627
810255P-5MG04061835185641