05-633
Anti-BrdU Antibody, clone BU-1
culture supernatant, clone BU-1, Upstate®
Synonym(s):
Anti-BrdU antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BU-1, monoclonal
Technique(s):
immunocytochemistry: suitable
Application:
ICC
Citations:
14
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
BU-1, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
isotype
IgG2a
shipped in
wet ice
target post-translational modification
unmodified
General description
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
none given
Immunogen
5-iodouridine:ovalbumin conjugate
Application
Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
Biochem/physiol Actions
5-Bromo-2-deoxyuridine
Physical form
4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.
Analysis Note
A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
高风险级别生物产品-Merck
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Immunofluorescence labeling indices in myeloma and related monoclonal gammopathies.
Greipp, P R, et al.
Mayo Clinic Proceedings. Mayo Clinic, 62, 969-977 (1987)
Models of S-phase determination in lymphomas from flow cytometric DNA content histograms: comparison with the bromodeoxyuridine labeling index.
Beauregard, P, et al.
Hematologic Pathology, 5, 177-183 (1991)
Macrophages and dendritic cells as actors in the immune reaction of classical Hodgkin lymphoma.
Tudor, CS; Bruns, H; Daniel, C; Distel, LV; Hartmann, A; Gerbitz, A; Buettner, MJ
Testing null