05-710
Anti-NG2 Antibody, clone 132.38
clone 132.38, Upstate®, from mouse
Synonym(s):
Anti-CSPG4A, Anti-HMW-MAA, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
132.38, monoclonal
Species reactivity:
rat, mouse
Application:
IHC, WB
Citations:
27
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
132.38, monoclonal
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
General description
~270-300 kDa
Immunogen
HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222
Application
Anti-NG2 Antibody, clone 132.38 detects level of NG2 & has been published & validated for use in IH & WB.
Cross-reactivity expected with mouse.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Biochem/physiol Actions
Recognizes NG2.
Physical form
Format: Purified
Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Protein G Purified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Brain tissue
Brain tissue
routinely evaluated by immunoblot on whole rat brain preparation
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sandra Stelzer et al.
BMC neuroscience, 11, 27-27 (2010-02-27)
Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability. So far
J M Levine et al.
Trends in neurosciences, 24(1), 39-47 (2001-02-13)
Adult oligodendrocyte precursor cells (OPCs) make up around 5-8% of the glial cell population in the CNS. Their function in the undamaged CNS is largely unknown, but their processes are in contact with nodes of Ranvier and synapses, suggesting a
Yvonne M Ughrin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(1), 175-186 (2003-01-07)
The NG2 chondroitin sulfate proteoglycan, an integral membrane proteoglycan, inhibits axon growth from cerebellar granule neurons and dorsal root ganglia (DRG) neurons in vitro. The extracellular domain of the NG2 core protein contains three subdomains: an N-terminal globular domain (domain