06-284
Anti-Nitrotyrosine Antibody
Upstate®, from rabbit
Synonym(s):
Anti-Nitrotyrosine, Detection Antibody, Nitrotyrosine Detection Antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Technique(s):
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
Application:
ICC, IHC, IP, WB
Citations:
318
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
General description
Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.
Predicted MW for nitrated-BSA: 85 kDa
Immunogen
Nitrated KLH
Application
- Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected in fixed and permeabilized A-431 cells treated with 5mM peroxynitrite for 15 minutes.
- Immunohistochemistry Applications: A representative lot detected Nitrotyrosine in human colon cancer, human Alzheimer′s brain and human diabetic kidney tissue sections.
- Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Research Sub Category
Neurotransmitters & Receptors
Neurodegenerative Diseases
Neurotransmitters & Receptors
Neurodegenerative Diseases
This rabbit polyclonal Anti-Nitrotyrosine, Cat. No. 06-284 is tested for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, for the detection of Nitrotyrosine.
Biochem/physiol Actions
Recognizes proteins which are nitrated.
Physical form
Format: Purified
ImmunoAffinity Purified
Purified Rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, 0.05% sodium azide, and 10 mg/mL BSA
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
routinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Reactive nitrogen species contribute to blood-labyrinth barrier disruption in suppurative labyrinthitis complicating experimental pneumococcal meningitis in the rat
Kastenbauer, S., et al
Brain Research, 904, 208-217 (2001)
Free 3-nitrotyrosine causes striatal neurodegeneration in vivo
Mihm, M. J., et al
The Journal of Neuroscience, 21, RC149-RC149 (2001)
A Perner et al.
Gut, 49(3), 387-394 (2001-08-21)
Luminal nitric oxide (NO) is greatly increased in the colon of patients with collagenous and ulcerative colitis. To define the source and consequence of enhanced NO production we have studied expression of NO synthase (NOS) isoforms and nitrotyrosine in mucosal
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 06-284 | 08436037124471 |