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06-333

Anti-MAPK1/ERK2 Antibody

Upstate, rabbit polyclonal

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, human, rat
Application:
IP, WB
Citations:
22
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Product Name

Anti-MAP Kinase 2/Erk2 Antibody, Upstate®, from rabbit

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_002745

UniProt accession no.

P28482

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAPKAPK2(9261)
mouse ... Mapkapk2(17164)
rat ... Mapkapk2(289014)

General description

42kDa

Immunogen

Recombinant mouse ERK2

Application

Anti-MAP Kinase 2/Erk2 Antibody detects level of MAP Kinase 2/Erk2 & has been published & validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

Biochem/physiol Actions

MAP Kinase encoded by the mapk gene

Physical form

0.02M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide
Format: Purified
Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on mouse 3T3/A31 fibroblasts, human A431 carcinoma cells or rat L6 skeletal fibroblasts

Other Notes

Replaces: 04-349

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dual phosphorylation and autophosphorylation in mitogen-activated protein (MAP) kinase activation.
Her, J H, et al.
The Biochemical Journal, 296 ( Pt 1), 25-31 (1993)
F Kiefer et al.
Molecular and cellular biology, 18(7), 4209-4220 (1998-06-25)
The cytoplasmic protein tyrosine kinase Syk has two amino-terminal SH2 domains that engage phosphorylated immunoreceptor tyrosine-based activation motifs in the signaling subunits of immunoreceptors. Syk, in conjunction with Src family kinases, has been implicated in immunoreceptor signaling in both lymphoid
Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth.
Jennifer D Cannon,Mary Cherian-Shaw,Tara Lovekamp-Swan,Charles L Chaffin
Molecular and cellular endocrinology null
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