06-536
Anti-Bak Antibody, NT
Upstate®, from rabbit
Synonym(s):
Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like 7 protein, pro-apoptotic protein BAK
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, IP, WB
Citations:
153
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... BAK1(578)
General description
28 kDa
Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.
Immunogen
Epitope: N-terminus
Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.
Application
Detect Bak using this Anti-Bak Antibody, NT validated for use in IC, IH, IP & WB.
Immunohistochemistry (Paraffin):
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Biochem/physiol Actions
Specific for Bak, MW ~28 kDa.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Evaluated by Western Blot on HEK293 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-433
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
CXCR4 chemokine receptor signaling induces apoptosis in acute myeloid leukemia cells via regulation of the Bcl-2 family members Bcl-XL, Noxa, and Bak.
Kremer, Kimberly N, et al.
The Journal of Biological Chemistry, 288, 22899-22914 (2013)
Bcl-xES, a BH4- and BH2-containing antiapoptotic protein, delays Bax oligomer formation and binds Apaf-1, blocking procaspase-9 activation.
Schmitt, E; Paquet, C; Beauchemin, M; Bertrand, R
Oncogene null
Evidence for involvement of BH3-only proapoptotic members in adenovirus-induced apoptosis.
Subramanian, T; Vijayalingam, S; Lomonosova, E; Zhao, LJ; Chinnadurai, G
Journal of virology null