Anti-c-Myc Tag Antibody

Myc proto-oncogene protein (UniProt: P01106; also known as Class E basic helix-loop-helix protein 39 (bHLHe39), Proto-oncogene c-Myc, Transcription factor p64) is encoded by the MYC (also known as BHLHE39) gene (Gene ID: 4609) in human. The Myc-tag is a short peptide sequence derived from the c-Myc protein. It is commonly used as an epitope tag in molecular biology. It is composed of 10 amino acids (EQKLISEEDL) and is fused to proteins of interest to facilitate their detection and purification. The Myc-tag functions as a marker that can be recognized by specific antibodies, allowing researchers to track the expression, localization, and interaction of tagged proteins in various experimental contexts.

Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.

Epitope: a.a. 409-420

Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).

Anti-Myc Tag, Cat. No. 06-549, is a rabbit polyclonal antibody that detects Myc-tagged proteins and is tested for use in Western Blotting and Immunocytochemistry.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Tested Applications Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Myc-tagged PP2A A subunit in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Other species cross-reactivity not tested.

This rabbit polyclonal antibody detects human Myc and Myc-tagged recombinant proteins.

Format: Purified

Protein A chromatography

Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Evaluated by Western Blotting in NIH/3T3 cells transfected with a Myc-tagged PP2A A subunit. Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected the Myc-tag in NIH/3T3 cells transfected with Myc-tagged PP2A A subunit.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.