Anti-mGluR7 Antibody

Metabotropic glutamate receptor 7 (UniProt: Q14831; also known as mGluR7) is encoded by the GRM7 (also known as GPRC1G, MGLUR7) gene (Gene ID: 2917) in human. GRM7 gene is mapped to human chromosome 3p26. L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. GRM7 belongs to Group III, and members of group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. mGluR7 is a multi-pass membrane protein and is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein (aa 35-915). mGluR7 is expressed in several parts of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. It is located in the presynaptic zone of the synaptic cleft of glutamatergic synapses. Five isoforms of mGluR7 have been described that are produced by alternative splicing. The gene coding MGLUR7 is associated with autism and also participates in schizophrenia and bipolar disorder. Dysregulation of mGluR7 has been implicated in several neuropsychiatric disorders, including anxiety, depression, and fragile X syndrome (FXS), where its modulation by allosteric agents like AMN082 has shown therapeutic potential. Studies suggest that drugs targeting mGluR7: mGluR7 agonists, antagonists, and allosteric modulators may play a role as promising agents for the treatment of central nervous system disorders. (Ref.: Li P., et al. (2024). Ageing Res Rev. 102;102554; Palazzo E., et al. (2016). Curr Neuropharmacol. 14(5); 504-513).

Target molecular weight ~ 97 kDa observed; 102.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal cytoplasmic domain of human mGluR7.

Anti-mGluR7, Cat. No. 07-239, is a rabbit polyclonal antibody that detects mGluR7 and is tested for use in Western Blotting and Dot Blot.

Research Category
Neuroscience

Tested Applications

Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected synthetic mGluR7.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

This polyclonal antibody specifically detects mGluR7. It targets an epitope within 14 amino acids from the C-terminal cytoplasmic domain.

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected mGluR7 in rat brain microsomal preparation.

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