07-468
Anti-HuR Antibody
Upstate®, from rabbit
Synonym(s):
ELAV-like protein 1, Hu-antigen R
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IP, WB
Citations:
13
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ELAVL1(1994)
General description
HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA binding protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK..
~36 kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 1-13 of human HuR.
Application
Immunoprecipitation Analysis:
10 µg from a representative lot detected HuR in RIPA lysate.
Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected HuR in HeLa and NIH/3T3 cell lysates.
Immunohistochemistry Analysis:
This antibody has been reported by an independent laboratory to detect HuR in rabbit placental tissue.
10 µg from a representative lot detected HuR in RIPA lysate.
Immunocytochemistry Analysis:
A 1:500 dilution from a representative lot detected HuR in HeLa and NIH/3T3 cell lysates.
Immunohistochemistry Analysis:
This antibody has been reported by an independent laboratory to detect HuR in rabbit placental tissue.
Anti-HuR Antibody is a Rabbit Polyclonal Antibody for detection of HuR also known as ELAV-like protein 1, Hu-antigen R & has been validated in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
RNA Binding Protein (RBP)
Transcription Factors
RNA Binding Protein (RBP)
Biochem/physiol Actions
HuR
Physical form
Format: Purified
See datasheet for exact formulation.
See datasheet for specific purification method.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Western Blot Analysis: Evaluated by Western Blot in 3T3/A31 cell lysate using 1 µg/mL of antibody and 10 µg of lysate.
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protein kinase C? mediates MCP-1 mRNA stabilization in vascular smooth muscle cells.
Bin Liu,Latika Dhawan,Burns C Blaxall,Mark B Taubman
Molecular and Cellular Biochemistry null
Tianke Chen et al.
Cell death & disease, 11(10), 822-822 (2020-10-04)
Hepatocellular carcinoma (HCC) is a major leading cause of cancer-related death worldwide. Alpha fetoprotein (AFP) is reactivated in a majority of hepatocellular carcinoma (HCC) and associated with poor patient outcomes. Although increasing evidence has shown that AFP can regulate HCC
Amanda C Vreeland et al.
Molecular and cellular biology, 34(12), 2135-2146 (2014-04-02)
The RNA-binding protein HuR binds at 3' untranslated regions (UTRs) of target transcripts, thereby protecting them against degradation. We show that HuR directly interacts with cellular retinoic acid-binding protein 2 (CRABP2), a protein known to transport RA from the cytosol