07-641
Anti-MAP2K1/MEK1 Antibody
Upstate, rabbit polyclonal
Synonym(s):
Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
IP, WB
Citations:
34
Product Name
Anti-MEK1 Antibody, Upstate®, from rabbit
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MAP2K1(5604)
General description
45kDa
Immunogen
Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.
Application
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.
Biochem/physiol Actions
MEK1
Predicted to cross-react with rabbit based on sequence homology.
Physical form
70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified
Preparation Note
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.
Other Notes
Replaces: 04-376
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors.
Kang, Z; Webster Marketon, JI; Johnson, A; Sternberg, EM
Journal of Molecular Biology null
Shihui Liu et al.
The Journal of biological chemistry, 283(1), 529-540 (2007-11-03)
Anthrax lethal toxin (LT), a virulence factor secreted by Bacillus anthracis, is selectively toxic to human melanomas with the BRAF V600E activating mutation because of its proteolytic activities toward the mitogen-activated protein kinase kinases (MEKs). To develop LT variants with
Jason M Warfel et al.
Toxins, 3(10), 1278-1293 (2011-11-10)
Systemic anthrax disease is characterized by vascular leakage pathologies. We previously reported that anthrax lethal toxin (LT) induces human endothelial barrier dysfunction in a cell death-independent manner with actin stress fiber formation and disruption of adherens junctions (AJs). In the