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1.08488

Urea

Name: Urea
Brand: MM

for biochemistry

Synonym(s):

Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide

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About This Item

Linear Formula:

NH₂CONH₂

CAS Number:

57-13-6

Molecular Weight:

60.06

UNSPSC Code:

12352111

NACRES:

NA.21

EC Index Number:

200-315-5

MDL number:

MFCD00008022

Beilstein/REAXYS Number:

635724

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Properties

vapor pressure

<0.1 hPa ( 20 °C)

Quality Level

100

form

solid

potency

8471 mg/kg LD₅₀, oral (Rat), 8200 mg/kg LD₅₀, skin (Rat)

technique(s)

titration: suitable

pH

9 (20 °C, 100 g/L in H₂O)

solubility

1000 g/L

density

1.335 g/mL at 25 °C (lit.)

bulk density

720‑760 kg/m³

storage temp.

10-30°C

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Analysis Note

Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance: Colorless crystals or white crystalline powder.
Appearance of solution (100 g/l, water): Clear and colorless.
Melting point (DSC (differential scanning calorimetry)): 132 - 135 °C
UV-Absorption (260 nm; 8 mol/l; 1 cm; water): ≤ 0.1
UV-Absorption (280 nm; 8 mol/l; 1 cm; water): ≤ 0.1
Cyanide (CN): ≤ 0.000001 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Fe (Iron): ≤ 0.00002 %
NH₄ (Ammonium): ≤ 0.00001 %
Biuret: ≤ 0.05 %
Sulfated ash (800 °C): ≤ 0.01 %

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c