1.12018
Albumin fraction V
(from bovine serum) for biochemistry
Synonym(s):
Albumin fraction V, Bovine serum albumin, Serum albumin
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About This Item
CAS Number:
MDL number:
NACRES:
NA.21
EC Index Number:
292-322-5
UNSPSC Code:
12352202
Biological source:
bovine
Form:
solid
Technique(s):
immunoassay: suitable, western blot: suitable
Concentration:
≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
biological source
bovine
form
solid
concentration
≥97% protein (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
technique(s)
immunoassay: suitable, western blot: suitable
pH
6.8-7.2 (20 °C, 1 g/L in H2O)
solubility
1 g/L
bulk density
150 kg/m3
storage temp.
2-8°C
General description
Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.
Application
Albumin fraction V has been used:
- as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
- as a blocking agent in western blot analysis
- as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids.
Analysis Note
Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.
Sebastian Ivens et al.
Brain : a journal of neurology, 130(Pt 2), 535-547 (2006-11-24)
It has long been recognized that insults to the cerebral cortex, such as trauma, ischaemia or infections, may result in the development of epilepsy, one of the most common neurological disorders. Human and animal studies have suggested that perturbations in
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1120180100 | 04022536138367 |
| 1120180025 | 04022536138350 |
| 1120180500 | 04022536712291 |