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Merck
CN

1.25195

Cellvento® 4HEK Powder

Chemically defined, powder, suitable (for HEK293 cell culture)

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About This Item

UNSPSC Code:
41106213
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Quality Level

description

shipped with cooling pack.

Dry powder medium must be supplemented with 2.5 g/L Sodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mM L-Glutamine

product line

Cellvento®

form

dry powder

technique(s)

cell culture | mammalian: suitable

suitability

suitable (for HEK293 cell culture)

storage temp.

2-8°C

General description

Cellvento® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Dry powder medium must be supplemented with 2.5 g/LSodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mML-Glutamine.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany


pictograms

Exclamation mark

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Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.

The current status and future manufacturing of AAV-based gene therapies is discussed in this podcast transcript, including how to streamline large-scale manufacturing.

Upstream gene therapy optimization maximizes viral vector titers; partner with technology experts for HEK293, HEK293T, and Sf9 cell solutions.

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