126626
Bovine Serum Albumin
30% Sterile-Filtered Aqueous Solution, Preservative-Free
Synonym(s):
Albumin, Bovine Serum, 30% Sterile-Filtered Aqueous Solution, Preservative-Free
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About This Item
Assay:
≥96% (agarose gel electrophoresis)
Form:
liquid
Technique(s):
immunohistochemistry: suitable
Product Name
Albumin, Bovine Serum, 30% Sterile-Filtered Aqueous Solution, Preservative-Free,
description
Merck USA index - 14, 8468
Quality Level
assay
≥96% (agarose gel electrophoresis)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
immunohistochemistry: suitable
color
pale amber
shipped in
ambient
storage temp.
2-8°C
General description
Albumin, Bovine Serum, 30% Sterile-Filtered Aqueous Solution, Preservative-Free is used as a stabilizing agent and carrier protein.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds. It is a member of the serum albumin family and has three domains with two sub-domains each.
Application
Albumin, Bovine Serum, 30% Sterile-Filtered Aqueous Solution, Preservative-Free has been used as a component of fluorescence-activated cell sorting (FACS) buffer to obtain a HaloTag CRISPR clone using FACS.
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is a critical component of cell culture media. It is useful for embryonic stem cells (hESC) differentiation and helps to transport drugs, hormones, and fatty acids. BSA works as a blocking agent in enzyme-linked immunosorbent assay (ELISA).
Physical form
Supplied as a 30% sterile-filtered solution in water, preservative-free.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 3
Certificates of Analysis (COA)
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Elizabeth A Caine et al.
Current protocols in pharmacology, 91(1), e81-e81 (2020-12-18)
To assess the role of a protein, protein loss phenotypic studies can be used, most commonly through mutagenesis RNAi or CRISPR knockout. Such studies have been critical for the understanding of protein function and the identification of putative therapeutic targets
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
Tamara Topală et al.
Clujul medical (1957), 87(4), 215-219 (2014-01-01)
The continuous search for new molecules with therapeutic abilities has led to the synthesis and characterization of a large number of metal complexes, proven to exhibit potential as pharmacological agents through their antibacterial, antiviral, antifungal and antineoplastic properties. As serum
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 126626-50ML | 04055977222739 |