14-392
Green Fluorescent Protein
Synonym(s):
GFP
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid
Assay:
>70%
Recombinant:
expressed in E. coli
Mol wt:
Mw ~28 kDa
General description
N-terminal HIS-tagged fusion protein corresponding to full-length Green Fluorescent Protein, expressed in E. coli. Green fluorescent protein (GFP) is a cytoplasmic protein found in jellyfish Aequorea Victoria.
Biochem/physiol Actions
Green fluorescent protein (GFP) is genetically fused to many proteins in various species to generate non-reactive chimeras which are known to preserve their original biological activity as well as the fluorescent properties of native GFP. GFP acts as a reporter for gene expression and as a fusion tag for protein localization studies in live cells. It serves as a spectroscopic and microscopic probe to understand the biological functions of various systems. GFP is highly stable and can serve as a tool for in vitro studies.
Physical form
PBS containing 20% glycerol.
Preparation Note
1 year at -20°C
Analysis Note
Routinely evaluated as a standard on protein gels and western immunoblots, for calibration of fluorometers, and microinjection into cells and tissues.
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
J L Casey et al.
Protein engineering, 13(6), 445-452 (2000-07-06)
We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). GFP is a
Elisa M York et al.
Biomedical optics express, 10(9), 4381-4394 (2019-10-01)
Autofluorescence of endogenous molecules can provide valuable insights in both basic research and clinical applications. One such technique is fluorescence lifetime imaging (FLIM) of NAD(P)H, which serves as a correlate of glycolysis and electron transport chain rates in metabolically active
Primary structure of the Aequorea victoria green-fluorescent protein.
Prasher, D C, et al.
Gene, 111, 229-233 (1992)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 14-392 | 04053252515590 |