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Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):

Agarose ChIP Kit, ChIP, Chromatin enrichment, chromatin immunoprecipitation

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.41
eCl@ss:
32161000
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Quality Level

100

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Analysis Note

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Other Notes

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

target_organs

Respiratory Tract

wgk

WGK 3

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - ED ENV 1 - Eye Irrit. 2 - STOT RE 2 Inhalation

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
17-29508436037124679